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3rd generation lentiviral packaging kit  (Applied Biological Materials Inc)

 
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    Applied Biological Materials Inc 3rd generation lentiviral packaging kit
    (A) Representative images from coculture demonstrating direct CA-MSC to TC mitochondrial transfer. CA-MSC mitochondria labeled with <t>lentiviral</t> COX8-GFP (green), while endogenous TC mitochondria are COX8-dsRed (orange). (i) Maximum projection image of representative coculture z stack. scale bar, 50 μm. (ii) Three-dimensional (3D) rendering of a subset of (i) showing TC +mitoTrans cell and (iii) representative z slices. (B) Transfer of GFP-CA-MSC mitochondria to TCs quantified using flow cytometry. Six individual patient (pt)-derived CA-MSCs are represented (i) and three individual patient-derived CA-MSCs donating to three individual ovarian cancer cell lines are represented (ii). (C) CA-MSC to TC mitochondrial transfer under indirect coculture vs. direct coculture ( n = 5 individual experiments). (D) Comparison of CA-MSCs’ and normal MSCs’ (derived from patients without cancer) ability to transfer mitochondria to TCs ( n = 3 individual experiments). (E) Change in CA-MSC to TC mitochondrial transfer under adherent vs. non-adherent conditions ( n = 5 individual experiments). (F) Change in CA-MSC to TC mitochondrial transfer under normoxic (21% O 2 ) vs. hypoxic (1% O 2 ) conditions ( n = 3 individual experiments). (G) CA-MSC mitochondrial transfer quantified comparing donation to TCs with the highest active mitochondrial content (top 20%, mito rich) and lowest active mitochondrial content (lower 20%, mito poor), as assessed by MitoTracker Deep Red staining. In (B)–(D), CA-MSCs were cocultured, resulting in a 10:1 TC:CA-MSC ratio. * p < 0.05. Data are presented as the mean ± SEM; displayed p values obtained using Student’s t test.
    3rd Generation Lentiviral Packaging Kit, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3rd+generation+lentiviral+packaging+kit/pmc11420855-369-16-20?v=Applied+Biological+Materials+Inc
    Average 90 stars, based on 1 article reviews
    3rd generation lentiviral packaging kit - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Carcinoma-associated mesenchymal stem cells promote ovarian cancer heterogeneity and metastasis through mitochondrial transfer"

    Article Title: Carcinoma-associated mesenchymal stem cells promote ovarian cancer heterogeneity and metastasis through mitochondrial transfer

    Journal: Cell reports

    doi: 10.1016/j.celrep.2024.114551

    (A) Representative images from coculture demonstrating direct CA-MSC to TC mitochondrial transfer. CA-MSC mitochondria labeled with lentiviral COX8-GFP (green), while endogenous TC mitochondria are COX8-dsRed (orange). (i) Maximum projection image of representative coculture z stack. scale bar, 50 μm. (ii) Three-dimensional (3D) rendering of a subset of (i) showing TC +mitoTrans cell and (iii) representative z slices. (B) Transfer of GFP-CA-MSC mitochondria to TCs quantified using flow cytometry. Six individual patient (pt)-derived CA-MSCs are represented (i) and three individual patient-derived CA-MSCs donating to three individual ovarian cancer cell lines are represented (ii). (C) CA-MSC to TC mitochondrial transfer under indirect coculture vs. direct coculture ( n = 5 individual experiments). (D) Comparison of CA-MSCs’ and normal MSCs’ (derived from patients without cancer) ability to transfer mitochondria to TCs ( n = 3 individual experiments). (E) Change in CA-MSC to TC mitochondrial transfer under adherent vs. non-adherent conditions ( n = 5 individual experiments). (F) Change in CA-MSC to TC mitochondrial transfer under normoxic (21% O 2 ) vs. hypoxic (1% O 2 ) conditions ( n = 3 individual experiments). (G) CA-MSC mitochondrial transfer quantified comparing donation to TCs with the highest active mitochondrial content (top 20%, mito rich) and lowest active mitochondrial content (lower 20%, mito poor), as assessed by MitoTracker Deep Red staining. In (B)–(D), CA-MSCs were cocultured, resulting in a 10:1 TC:CA-MSC ratio. * p < 0.05. Data are presented as the mean ± SEM; displayed p values obtained using Student’s t test.
    Figure Legend Snippet: (A) Representative images from coculture demonstrating direct CA-MSC to TC mitochondrial transfer. CA-MSC mitochondria labeled with lentiviral COX8-GFP (green), while endogenous TC mitochondria are COX8-dsRed (orange). (i) Maximum projection image of representative coculture z stack. scale bar, 50 μm. (ii) Three-dimensional (3D) rendering of a subset of (i) showing TC +mitoTrans cell and (iii) representative z slices. (B) Transfer of GFP-CA-MSC mitochondria to TCs quantified using flow cytometry. Six individual patient (pt)-derived CA-MSCs are represented (i) and three individual patient-derived CA-MSCs donating to three individual ovarian cancer cell lines are represented (ii). (C) CA-MSC to TC mitochondrial transfer under indirect coculture vs. direct coculture ( n = 5 individual experiments). (D) Comparison of CA-MSCs’ and normal MSCs’ (derived from patients without cancer) ability to transfer mitochondria to TCs ( n = 3 individual experiments). (E) Change in CA-MSC to TC mitochondrial transfer under adherent vs. non-adherent conditions ( n = 5 individual experiments). (F) Change in CA-MSC to TC mitochondrial transfer under normoxic (21% O 2 ) vs. hypoxic (1% O 2 ) conditions ( n = 3 individual experiments). (G) CA-MSC mitochondrial transfer quantified comparing donation to TCs with the highest active mitochondrial content (top 20%, mito rich) and lowest active mitochondrial content (lower 20%, mito poor), as assessed by MitoTracker Deep Red staining. In (B)–(D), CA-MSCs were cocultured, resulting in a 10:1 TC:CA-MSC ratio. * p < 0.05. Data are presented as the mean ± SEM; displayed p values obtained using Student’s t test.

    Techniques Used: Labeling, Flow Cytometry, Derivative Assay, Comparison, Staining



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    3rd generation lentiviral packaging kit  (Applied Biological Materials Inc)
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    Applied Biological Materials Inc 3rd generation lentiviral packaging kit
    (A) Representative images from coculture demonstrating direct CA-MSC to TC mitochondrial transfer. CA-MSC mitochondria labeled with <t>lentiviral</t> COX8-GFP (green), while endogenous TC mitochondria are COX8-dsRed (orange). (i) Maximum projection image of representative coculture z stack. scale bar, 50 μm. (ii) Three-dimensional (3D) rendering of a subset of (i) showing TC +mitoTrans cell and (iii) representative z slices. (B) Transfer of GFP-CA-MSC mitochondria to TCs quantified using flow cytometry. Six individual patient (pt)-derived CA-MSCs are represented (i) and three individual patient-derived CA-MSCs donating to three individual ovarian cancer cell lines are represented (ii). (C) CA-MSC to TC mitochondrial transfer under indirect coculture vs. direct coculture ( n = 5 individual experiments). (D) Comparison of CA-MSCs’ and normal MSCs’ (derived from patients without cancer) ability to transfer mitochondria to TCs ( n = 3 individual experiments). (E) Change in CA-MSC to TC mitochondrial transfer under adherent vs. non-adherent conditions ( n = 5 individual experiments). (F) Change in CA-MSC to TC mitochondrial transfer under normoxic (21% O 2 ) vs. hypoxic (1% O 2 ) conditions ( n = 3 individual experiments). (G) CA-MSC mitochondrial transfer quantified comparing donation to TCs with the highest active mitochondrial content (top 20%, mito rich) and lowest active mitochondrial content (lower 20%, mito poor), as assessed by MitoTracker Deep Red staining. In (B)–(D), CA-MSCs were cocultured, resulting in a 10:1 TC:CA-MSC ratio. * p < 0.05. Data are presented as the mean ± SEM; displayed p values obtained using Student’s t test.
    3rd Generation Lentiviral Packaging Kit, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3rd+generation+lentiviral+packaging+kit/pmc11420855-369-16-20?v=Applied+Biological+Materials+Inc
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    3rd generation lenti vpak packaging kit  (OriGene)
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    (A) Representative images from coculture demonstrating direct CA-MSC to TC mitochondrial transfer. CA-MSC mitochondria labeled with <t>lentiviral</t> COX8-GFP (green), while endogenous TC mitochondria are COX8-dsRed (orange). (i) Maximum projection image of representative coculture z stack. scale bar, 50 μm. (ii) Three-dimensional (3D) rendering of a subset of (i) showing TC +mitoTrans cell and (iii) representative z slices. (B) Transfer of GFP-CA-MSC mitochondria to TCs quantified using flow cytometry. Six individual patient (pt)-derived CA-MSCs are represented (i) and three individual patient-derived CA-MSCs donating to three individual ovarian cancer cell lines are represented (ii). (C) CA-MSC to TC mitochondrial transfer under indirect coculture vs. direct coculture ( n = 5 individual experiments). (D) Comparison of CA-MSCs’ and normal MSCs’ (derived from patients without cancer) ability to transfer mitochondria to TCs ( n = 3 individual experiments). (E) Change in CA-MSC to TC mitochondrial transfer under adherent vs. non-adherent conditions ( n = 5 individual experiments). (F) Change in CA-MSC to TC mitochondrial transfer under normoxic (21% O 2 ) vs. hypoxic (1% O 2 ) conditions ( n = 3 individual experiments). (G) CA-MSC mitochondrial transfer quantified comparing donation to TCs with the highest active mitochondrial content (top 20%, mito rich) and lowest active mitochondrial content (lower 20%, mito poor), as assessed by MitoTracker Deep Red staining. In (B)–(D), CA-MSCs were cocultured, resulting in a 10:1 TC:CA-MSC ratio. * p < 0.05. Data are presented as the mean ± SEM; displayed p values obtained using Student’s t test.
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    https://www.bioz.com/product/3rd+generation+lentiviral+packaging+kit/pm30542505-151-14-22?v=OriGene
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    Image Search Results


    (A) Representative images from coculture demonstrating direct CA-MSC to TC mitochondrial transfer. CA-MSC mitochondria labeled with lentiviral COX8-GFP (green), while endogenous TC mitochondria are COX8-dsRed (orange). (i) Maximum projection image of representative coculture z stack. scale bar, 50 μm. (ii) Three-dimensional (3D) rendering of a subset of (i) showing TC +mitoTrans cell and (iii) representative z slices. (B) Transfer of GFP-CA-MSC mitochondria to TCs quantified using flow cytometry. Six individual patient (pt)-derived CA-MSCs are represented (i) and three individual patient-derived CA-MSCs donating to three individual ovarian cancer cell lines are represented (ii). (C) CA-MSC to TC mitochondrial transfer under indirect coculture vs. direct coculture ( n = 5 individual experiments). (D) Comparison of CA-MSCs’ and normal MSCs’ (derived from patients without cancer) ability to transfer mitochondria to TCs ( n = 3 individual experiments). (E) Change in CA-MSC to TC mitochondrial transfer under adherent vs. non-adherent conditions ( n = 5 individual experiments). (F) Change in CA-MSC to TC mitochondrial transfer under normoxic (21% O 2 ) vs. hypoxic (1% O 2 ) conditions ( n = 3 individual experiments). (G) CA-MSC mitochondrial transfer quantified comparing donation to TCs with the highest active mitochondrial content (top 20%, mito rich) and lowest active mitochondrial content (lower 20%, mito poor), as assessed by MitoTracker Deep Red staining. In (B)–(D), CA-MSCs were cocultured, resulting in a 10:1 TC:CA-MSC ratio. * p < 0.05. Data are presented as the mean ± SEM; displayed p values obtained using Student’s t test.

    Journal: Cell reports

    Article Title: Carcinoma-associated mesenchymal stem cells promote ovarian cancer heterogeneity and metastasis through mitochondrial transfer

    doi: 10.1016/j.celrep.2024.114551

    Figure Lengend Snippet: (A) Representative images from coculture demonstrating direct CA-MSC to TC mitochondrial transfer. CA-MSC mitochondria labeled with lentiviral COX8-GFP (green), while endogenous TC mitochondria are COX8-dsRed (orange). (i) Maximum projection image of representative coculture z stack. scale bar, 50 μm. (ii) Three-dimensional (3D) rendering of a subset of (i) showing TC +mitoTrans cell and (iii) representative z slices. (B) Transfer of GFP-CA-MSC mitochondria to TCs quantified using flow cytometry. Six individual patient (pt)-derived CA-MSCs are represented (i) and three individual patient-derived CA-MSCs donating to three individual ovarian cancer cell lines are represented (ii). (C) CA-MSC to TC mitochondrial transfer under indirect coculture vs. direct coculture ( n = 5 individual experiments). (D) Comparison of CA-MSCs’ and normal MSCs’ (derived from patients without cancer) ability to transfer mitochondria to TCs ( n = 3 individual experiments). (E) Change in CA-MSC to TC mitochondrial transfer under adherent vs. non-adherent conditions ( n = 5 individual experiments). (F) Change in CA-MSC to TC mitochondrial transfer under normoxic (21% O 2 ) vs. hypoxic (1% O 2 ) conditions ( n = 3 individual experiments). (G) CA-MSC mitochondrial transfer quantified comparing donation to TCs with the highest active mitochondrial content (top 20%, mito rich) and lowest active mitochondrial content (lower 20%, mito poor), as assessed by MitoTracker Deep Red staining. In (B)–(D), CA-MSCs were cocultured, resulting in a 10:1 TC:CA-MSC ratio. * p < 0.05. Data are presented as the mean ± SEM; displayed p values obtained using Student’s t test.

    Article Snippet: shRNA constructs targeting MIRO1/RHOT1 (HSH070029-LVRU6MP, GeneCopoeia, Rockville, MD) were packaged into lentiviral particles using a 3rd Generation Lentiviral Packaging Kit (Applied Biological Materials Inc., Richmond, BC).

    Techniques: Labeling, Flow Cytometry, Derivative Assay, Comparison, Staining